Ts'ebetso ea tlhahlobo ea lisampole tsa metsi e na le mefuta e mengata ea ts'ebeliso ho mahlale a bophelo le tlhahlobo ea tikoloho.Mosebetsing ona, re thehile photometer e kopanetsoeng le e theko e tlaase e thehiloeng ho li-capillaries tsa waveguide tsa tšepe (MCCs) bakeng sa boikemisetso bo matla ba ho monya.Tsela ea optical e ka eketsoa haholo, 'me e telele ho feta bolelele ba' mele ba MWC, hobane leseli le hasantsoeng ke marako a tšepe a boreleli a ka ba teng ka har'a capillary ho sa tsotelehe hore na ketsahalo e kae.Ho tsepamisa maikutlo ho tlase joalo ka 5.12 nM ho ka finyelloa ho sebelisoa li-reagents tse tloaelehileng tsa chromogenic ka lebaka la amplification e ncha e sa tloaelehang ea optical le ho fetola sampole ka potlako le ho lemoha tsoekere.
Photometry e sebelisoa haholo bakeng sa tlhahlobo ea lisampole tsa mokelikeli ka lebaka la bongata ba li-reagents tse fumanehang tsa chromogenic le lisebelisoa tsa semiconductor optoelectronic1,2,3,4,5.Ha ho bapisoa le tloaelo ea cuvette-based absorbance determination, liquid waveguide (LWC) capillaries reflect (TIR) ka ho boloka leseli la probe ka hare ho capillary1,2,3,4,5.Leha ho le joalo, ntle le ntlafatso e eketsehileng, tsela ea optical e haufi feela le bolelele ba 'mele oa LWC3.6,' me ho eketsa bolelele ba LWC ho feta 1.0 m ho tla hlokofatsoa ke khanya e matla ea leseli le kotsi e kholo ea li-bubble, joalo-joalo 3, 7. Mabapi le ho ea seleng e reriloeng e nang le mekhoa e mengata bakeng sa ntlafatso ea litsela tsa optical, moeli oa ho lemoha o ntlafatsoa feela ke karolo ea 2.5-8.9.
Hona joale ho na le mefuta e 'meli e meholo ea LWC, e leng Teflon AF capillaries (e nang le index ea refractive ea ~ 1.3 feela, e ka tlaase ho ea metsi) le silica capillaries e koahetsoeng ka Teflon AF kapa lifilimi tsa tšepe1,3,4.Ho finyella TIR ka har'a sehokelo pakeng tsa lisebelisoa tsa dielectric, lisebelisoa tse nang le index e tlase ea refractive le li-angles tse phahameng tsa khanya li hlokahala3,6,10.Mabapi le li-capillaries tsa Teflon AF, Teflon AF e phefumoloha ka lebaka la sebopeho sa eona se nang le masoba3,11 mme e khona ho monya lintho tse nyane lisampong tsa metsi.Bakeng sa li-capillaries tsa quartz tse koahetsoeng ka ntle le Teflon AF kapa tšepe, index ea refractive ea quartz (1.45) e phahame ho feta lisampole tse ngata tsa metsi (mohlala 1.33 bakeng sa metsi)3,6,12,13.Bakeng sa li-capillaries tse koahetsoeng ka filimi ea tšepe ka hare, thepa ea lipalangoang e 'nile ea ithutoa14,15,16,17,18, empa mokhoa oa ho roala o rarahane, holim'a filimi ea tšepe e na le mohaho o thata le o nang le porous4,19.
Ho feta moo, LWCs tsa khoebo (AF Teflon Coated Capillaries le AF Teflon Coated Silica Capillaries, World Precision Instruments, Inc.) li na le mefokolo e meng, joalo ka: bakeng sa liphoso..Molumo o moholo o shoeleng oa TIR3,10, (2) T-connector (ho hokahanya li-capillaries, fibers, le li-inlet/outlet tubes) o ka tšoara li-bubble tsa moea10.
Ka nako e ts'oanang, boikemisetso ba maemo a tsoekere bo bohlokoa haholo bakeng sa tlhahlobo ea lefu la tsoekere, lefu la ho thatafala ha sebete le lefu la kelello20.le mekhoa e mengata ea ho lemoha e kang photometry (ho kenyeletsoa spectrophotometry 21, 22, 23, 24, 25 le colorimetry pampiring 26, 27, 28), galvanometry 29, 30, 31, fluorometry 32, 33, 34, 35, polarimetry 3 holim'a plasmon resonance.37, Fabry-Perot cavity 38, electrochemistry 39 le capillary electrophoresis 40,41 joalo-joalo.Leha ho le joalo, boholo ba mekhoa ena e hloka lisebelisoa tse theko e boima, 'me ho fumanoa ha tsoekere ka bongata ba nanomolar e ntse e le phephetso (mohlala, bakeng sa litekanyo tsa photometric21, 22, 23, 24, 25, 26, 27, 28, khatello e tlaase ka ho fetisisa ea tsoekere).moeli o ne o le 30 nM feela ha li-nanoparticles tse putsoa tsa Prussia li ne li sebelisoa e le mohlala oa peroxidase).Litlhahlobo tsa tsoekere ea nanomolar hangata lia hlokahala bakeng sa lithuto tsa cellular tsa boemo ba limolek'hule joalo ka thibelo ea kholo ea mofetše oa tšoelesa ea senya42 le boits'oaro ba CO2 ba ho lokisa ba Prochlorococcus leoatleng.
Nako ea poso: Nov-26-2022